福建市場活動之——Bio-Rad 福建定量PCR實驗技術講座
2011-03-20
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Bio-Rad 福建 定量PCR講座具體時間安排
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歡迎廣大師生前來參加！
2011.3.29 福建農林菌類實驗室（伯樂 余小菁 陳洪亮）
09:00-10:00
MIQE Guidelines
Minimum Information for Publication of qPCR Experiments
10:00-10:30
Assay Design Considerations, Optimization and Validation
Primers and Probes Designs
Assay Validation
Gradient
10:30-10:45
Break
10:45-11:20
Sample Preparation & Quality Control
Biology & Chemistry
DNA & RNA Isolation
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11:20-12:00
q PCR Chemistry
Taqman
SYBR Green
Molecular Beacons
Dual-Oligo FRET Probes
12:00-13:30
Lauch
13:30-14:00
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Practical course
Use Ssofast Eva Green supermix to validate and optimize qPCR
14:00:14:30
Superior qPCR Reagent and plastic consumable from Bio-Rad
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By hongliangchen
14:30-15:30
Practical course data analysis
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Standard curve and melt curve
15:30-16:00
Break
16:00-16:30
Relative Quantifications: Data Management & Analysis Settings
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Housekeeping genes
Controls
16:30-17:00
Discussion
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